The second oldest unsolved homicide case in Alachua County happened on what was a very busy U.S. Highway in 1969. Before I-75, U.S. 441 was the major artery between Ocala and Gainesville. There was only one country store along that strip of highway. While the store has since been replaced by the subdivision, nothing has erased what happened there. TV 20's Paige Beck takes a look at the unsolved murder of Pearl Bartley.

Watch the video to your right for the full story.